DNA extraction from formalin-fixed Franciscana tissues

Rodríguez, D. and Bastida, R. and Olsson, P. E. (2002) DNA extraction from formalin-fixed Franciscana tissues. Latin American Journal of Aquatic Mammals, 1(1), pp. 123-128.

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Abstract

The present paper reports the extraction of DNA from formalin-fixed Pontoporia blainvillei tissues. Following the Vachot and Monerot (1996) protocol, fragmented DNA (300-700bp) was extracted from more than 95% of liver and muscle samples. DNA yield in liver samples was significantly higher than in muscle samples (4.574 ± 1.169mg DNA/mg versus 0.808 ± 0.297mg DNA/mg). Similar results were obtained from nine other species of cetaceans and five species of pinnipeds. It is of special interest to have a method that allows the utilisation of museum specimens not originally preserved for genetic studies, which may include rarely available, declining or extinct species. SPANISH: El presente trabajo reporta la extracción de ADN a partir de tejidos formolizados de Pontoporia blainvillei. Siguiendo el protocolo de Vachot y Monerot (1996) se pudo extraer ADN degradado (300-700pb) en más del 95% de las muestras de hígado y músculo analizadas. El rendimiento en ADN fue significativamente mayor en muestras de hígado que en muestras de músculo (4.574 ± 1.169mg DNA/mg tejido húmedo versus 0.808 ± 0.297mg DNA/mg tejido húmedo). Resultados similares se obtuvieron en otras nueve especies de Cetáceos y cinco de Pinnípedos. Resulta de gran interés contar con un método que permita la utilización de especímenes depositados en museos y que no hayan sido originalmente colectados para estudios genéticos, incluyendo especies de difícil obtención, en franca declinación o extintas.

Item Type: Article
Title: DNA extraction from formalin-fixed Franciscana tissues
Personal Creator/Author:
CreatorsEmail
Rodríguez, D.dhrodri@mdp.edu.ar
Bastida, R.
Olsson, P. E.
Corporate Creator/Author: ..
Refereed: Yes
Journal or Publication Title: Latin American Journal of Aquatic Mammals
Volume: 1
Number: 1
Page Range: pp. 123-128
Date: 2002
ISSN: 1676-7497
Additional Information: Special Issue No. 1, 2002
Subjects: Biology
Chemistry
Health
Oceanography
Item ID: 10035
Depositing User: Michelle Leonard
Date Deposited: 27 Sep 2012 17:06
Last Modified: 27 Sep 2012 17:06
URI: http://aquaticcommons.org/id/eprint/10035

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