An Improved molecular tool for distinguishing monoecious and dioedious Hydrilla

Madeira, Paul T. and Van, Thai K. and Center, Ted D. (2004) An Improved molecular tool for distinguishing monoecious and dioedious Hydrilla. Journal of Aquatic Plant Management, 42, pp. 28-32.

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Official URL: http://www.apms.org/japm/vol42/v42p28.pdf

Abstract

Two biotypes of hydrilla [Hydrilla verticillata(L.f.) Royle] occur in the United States, a dioecious type centered in the southeast and a monoecious type in the central Atlantic and northeastern states. Ecosystem managers need tools to distinguish the types as the ranges of each type expand and begin to overlap. A molecular tool using the randomly amplified polymorphic DNA (RAPD) procedure is available but its use is limited by a need for reference samples. We describe an alternative molecular tool which uses “universal primers” to sequence the trnL intron and trnL-F intergenic spacer of the chloroplast genome. This sequence yields three differences between the biotypes (two gaps and one single nucleotide polymorphism). A primer has been designed which ends in a gap that shows up only in the dioecious plant. A polymerase chain reaction (PCR) using this primer produces a product for the monoecious but not the dioecious plant.

Item Type: Article
Title: An Improved molecular tool for distinguishing monoecious and dioedious Hydrilla
Personal Creator/Author:
CreatorsEmail
Madeira, Paul T.
Van, Thai K.
Center, Ted D.
Refereed: Yes
Journal or Publication Title: Journal of Aquatic Plant Management
Volume: 42
Page Range: pp. 28-32
Date: 2004
Issuing Agency: Aquatic Plant Management Society, Inc.
Uncontrolled Keywords: Hydrilla verticillata; trnL intron; trnL-F intergenic spacer; sequencing.
Subjects: Biology
Item ID: 1672
Depositing User: Jennifer Walton
Date Deposited: 10 Nov 2008 15:33
Last Modified: 29 Sep 2011 20:14
URI: http://aquaticcommons.org/id/eprint/1672

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