Monitoring hydrilla using two RAPD procedures and the Nonindigenous Aquatic Species database

Madeira, Paul T. and Jacono, Collete C. and Van, Thai K. (2000) Monitoring hydrilla using two RAPD procedures and the Nonindigenous Aquatic Species database. Journal of Aquatic Plant Management, 38, pp. 33-40.

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Hydrilla ( Hydrilla verticillata (L.f.) Royle), an invasive aquatic weed, continues to spread to new regions in the United States. Two biotypes, one a female dioecious and the other monoecious have been identified. Management of the spread of hydrilla requires understanding the mechanisms of introduction and transport, an ability to map and make available information on distribution, and tools to distinguish the known U.S. biotypes as well as potential new introductions. Review of the literature and discussions with aquatic scientists and resource managers point to the aquarium and water garden plant trades as the primary past mechanism for the regional dispersal of hydrilla while local dispersal is primarily carried out by other mechanisms such as boat traffic, intentional introductions, and waterfowl. The Nonindigenous Aquatic Species (NAS) database is presented as a tool for assembling, geo-referencing, and making available information on the distribution of hydrilla. A map of the current range of dioecious and monoecious hydrilla by drainage is presented. Four hydrilla samples, taken from three discrete, non-contiguous regions (Pennsylvania, Connecticut, and Washington State) were examined using two RAPD assays. The first, generated using primer Operon G17, and capable of distinguishing the dioecious and monoecious U.S. biotypes, indicated all four samples were of the monoecious biotype. Results of the second assay using the Stoffel fragment and 5 primers, produced 111 markers, indicated that these samples do not represent new foreign introductions. The differences in the monoecious and dioecious growth habits and management are discussed.

Item Type: Article
Title: Monitoring hydrilla using two RAPD procedures and the Nonindigenous Aquatic Species database
Personal Creator/Author:
Madeira, Paul T.
Jacono, Collete C.
Van, Thai K.
Refereed: Yes
Journal or Publication Title: Journal of Aquatic Plant Management
Volume: 38
Page Range: pp. 33-40
Date: 2000
Issuing Agency: Aquatic Plant Management Society, Inc.
Uncontrolled Keywords: Randomly amplified polymorphic DNA, Hydrilla verticillata , monoecious, dioecious, spread, dispersal, Washington, Connecticut, Pennsylvania
Subjects: Management
Item ID: 1810
Depositing User: Stephanie Haas
Date Deposited: 12 Jan 2009 00:54
Last Modified: 29 Sep 2011 20:01

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