Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays

Vanersea, Mark W. and Litaker, R. Wayne and Marancik, Katrin E. and Hare, Jonathan A. and Walsh, Harvey J. and Lem, Siya and West, Melissa A. and Wyanski, David M. and Laban, Elisabeth H. and Tester, Patricia A. (2008) Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays. Fishery Bulletin, 106(2), pp. 183-193.

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Abstract

The identification of sea bass (Centropristis) larvae to species is difficult because of similar morphological characters, spawning times, and overlapping species ranges. Black sea bass (Centropristis striata) is an important fishery species and is currently considered to be overfished south of Cape Hatteras, North Carolina. We describe methods for identifying three species of sea bass larvae using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assays based on species-specific amplification of rDNA internal transcribed spacer regions. The assays were tested against DNA of ten other co-occurring reef fish species to ensure the assay's specificity. Centropristis larvae were collected on three cruises during cross-shelf transects and were used to validate the assays. Seventy-six Centropristis larva were assayed and 69 (91%) were identified successfully. DNA was not amplified from 5% of the larvae and identification was inconclusive for 3% of the larvae. Those assays can be used to identify sea bass eggs and larvae and will help to assess spawning locations, spawning times, and larval dispersal.

Item Type: Article
Title: Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays
Personal Creator/Author:
CreatorsEmail
Vanersea, Mark W.Mark.W.Vandersea@noaa.gov
Litaker, R. WayneWayne.Litaker@noaa.gov
Marancik, Katrin E.
Hare, Jonathan A.Jon.Hare@noaa.gov
Walsh, Harvey J.
Lem, Siya
West, Melissa A.
Wyanski, David M.
Laban, Elisabeth H.
Tester, Patricia A.
Journal or Publication Title: Fishery Bulletin
Volume: 106
Number: 2
Page Range: pp. 183-193
Date: 2008
Issuing Agency: United States National Marine Fisheries Service
Uncontrolled Keywords: sea basses; Centropristis spp.; genetic identification
Subjects: Ecology
Fisheries
Chemistry
Item ID: 2085
Depositing User: Patti M. Marraro
Date Deposited: 17 May 2009 18:39
Last Modified: 29 Sep 2011 19:41
URI: http://aquaticcommons.org/id/eprint/2085

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