Kammann, Ulrike and Bunke, Markus and Steinhart, Hans (2000) Anwendung von Fischzellkulturen in der Meeresforschung. Informationen für die Fischwirtschaft aus der Fischereiforschung, 47(1), pp. 48-51.
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Abstract
A large number of chemical pollutants can be found in the marine environment. So it is necessary to obtain informations about the toxic effects of this contaminant mixtures in general and especially on single cell level. We used an organic extract of a marine sediment from the North Sea to investigate its cyto- and genotoxicity with an in vitro system, the comet assay or single cell gel electrophoresis (SCGE). The comet assay can be applied for estimating genotoxic effects of chemicals on single cell level. First results confirm the sensitivity of this assay and its applicability in assessing genotoxic load in environmental samples. A permant cell line, the EPC (Ephithelioma papulosum cyprini) was used for the experiments. It was possible to demonstrate the suitability of this in vitro test system for assessing genotoxic and cytotoxic effects of marine sediment extracts on EPC cells.
| Item Type: | Article | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Title: | Anwendung von Fischzellkulturen in der Meeresforschung | ||||||||
| Personal Creator/Author: |
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| Refereed: | No | ||||||||
| Journal or Publication Title: | Informationen für die Fischwirtschaft aus der Fischereiforschung | ||||||||
| Volume: | 47 | ||||||||
| Number: | 1 | ||||||||
| Page Range: | pp. 48-51 | ||||||||
| Date: | 2000 | ||||||||
| ISSN: | 1437-5842 | ||||||||
| Contact Email Address: | ulrike.kammann@vti.bund.de | ||||||||
| Issuing Agency: | Bundesforschungsanstalt für Fischerei | ||||||||
| Additional Information: | Johann Heinrich von Thünen-Institute, Federal Research Institute for Rural Areas, Forestry and Fisheries began publishing the Informationen aus der Fischereiforschung = Information on Fishery research in 2010. | ||||||||
| Uncontrolled Keywords: | toxicity; marine fish cells; gentoxicity | ||||||||
| Subjects: | Ecology Environment Chemistry | ||||||||
| Item ID: | 3245 | ||||||||
| Depositing User: | Horst Bahl | ||||||||
| Date Deposited: | 07 May 2010 19:15 | ||||||||
| Last Modified: | 10 Nov 2012 15:39 | ||||||||
| URI: | http://aquaticcommons.org/id/eprint/3245 |
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