Preliminary study on the natural extenders for artificial breeding of African catfish Clarias gariepinus (Burchell, 1822)

Muchlisin, Z.A and Siti-Azizah, M.N. (2010) Preliminary study on the natural extenders for artificial breeding of African catfish Clarias gariepinus (Burchell, 1822). AACL Bioflux, 3(2), pp. 119-124.

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Abstract

The objective of the present study was to determine the most suitable extender and their respective dilution ratios for African catfish sperm for artificial induced breeding and cryopreservation purposes. Three natural extenders were tested i.e. coconut water, sugarcane water and soybean solutions, at three different levels of sperm to extender dilutions of 1:20, 1:30 and 1:40. While Ringer solution was used as a control Diluted sperm were fertilized with ready isolated eggs to assess the fertility and hatching rate at 0, 6 and 12 hour intervals. The results showed that the eggs hatched approximately 19 to 27 hours after fertilization. In general, the fertilization and hatching rates decreased with increasing dilution ratio. With respect to natural extenders, the coconut water showed the highest fertility and hatching rates at 1:20 dilution ratio. Therefore, coconut water at 1:20 dilution ratio was the optimal condition for African catfish spermatozoa among the natural extenders investigated.

Item Type: Article
Title: Preliminary study on the natural extenders for artificial breeding of African catfish Clarias gariepinus (Burchell, 1822)
Personal Creator/Author:
CreatorsEmail
Muchlisin, Z.Amuchlisinza@yahoo.com
Siti-Azizah, M.N.
Refereed: Yes
Journal or Publication Title: AACL Bioflux
Volume: 3
Number: 2
Page Range: pp. 119-124
Date: 2010
Additional Information: Syiah Kuala University; Universiti Sains malaysia
Subjects: Fisheries
Aquaculture
Item ID: 3649
Depositing User: Dr. Z.A. Muchlisin
Date Deposited: 07 Jul 2010 07:59
Last Modified: 10 Jan 2012 13:32
Related URLs:
URI: http://aquaticcommons.org/id/eprint/3649

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