On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]

Pyrina, I.L. and Mokeeva, N.P. (1966) On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]. Windermere, UK, Freshwater Biological Association, (FBA Translations (New Series), 39)

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Abstract

The original method, proposed by Yentsch (1957), of determination of chlorophyll directly in the cells, attracts attention by its simplicity. In order to measure the content of chlorophyll by this method, a determined volume of suspension of algae is filtered through a membrane filter. The latter is dried a little, clarified by immersion oil, clamped between two glasses, and spectrophotometrized. Extinction is read off at , wavelengths equal to 670 millimicrons (around the maximum absorption of chlorophyll a in the cell) and 750 millimicrons (correction for non- specific absorption and dispersion of light by particles of the preparation). The method of Yentsch was employed by the authors for determination of chlorophyll-a in samples of phytoplankton. They conclude that in spite of the simplicity and convenience of determination the method must be applied sufficiently carefully. It is more suitable for analysis of cultures of algae, where, non-specific absorption of light is insignificant.

Item Type: Monograph or Serial Issue
Title: On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]
Personal Creator/Author:
CreatorsEmail
Pyrina, I.L.
Mokeeva, N.P.
Series Name: FBA Translations (New Series)
Number: 39
Date: 1966
Publisher: Freshwater Biological Association
Place of Publication: Windermere, UK
Issuing Agency: Freshwater Biological Association
Additional Information: Translated from Russian into English
Uncontrolled Keywords: Algae; Chlorophylls; Methodology; Biofilters; Light absorption
Subjects: Biology
Ecology
Limnology
Item ID: 4794
Depositing User: Hardy B Schwamm
Date Deposited: 14 Feb 2011 12:55
Last Modified: 29 Sep 2011 15:52
Related URLs:
URI: http://aquaticcommons.org/id/eprint/4794

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