Pyrina, I.L. and Mokeeva, N.P. (1966) On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]. Windermere, UK, Freshwater Biological Association, (FBA Translations (New Series), 39)
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Abstract
The original method, proposed by Yentsch (1957), of determination of chlorophyll directly in the cells, attracts attention by its simplicity. In order to measure the content of chlorophyll by this method, a determined volume of suspension of algae is filtered through a membrane filter. The latter is dried a little, clarified by immersion oil, clamped between two glasses, and spectrophotometrized. Extinction is read off at , wavelengths equal to 670 millimicrons (around the maximum absorption of chlorophyll a in the cell) and 750 millimicrons (correction for non- specific absorption and dispersion of light by particles of the preparation). The method of Yentsch was employed by the authors for determination of chlorophyll-a in samples of phytoplankton. They conclude that in spite of the simplicity and convenience of determination the method must be applied sufficiently carefully. It is more suitable for analysis of cultures of algae, where, non-specific absorption of light is insignificant.
| Item Type: | Monograph or Serial issue | ||||||
|---|---|---|---|---|---|---|---|
| Title: | On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.] | ||||||
| Personal Creator/Author: |
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| Series Name: | FBA Translations (New Series) | ||||||
| Number: | 39 | ||||||
| Date: | 1966 | ||||||
| Publisher: | Freshwater Biological Association | ||||||
| Place of Publication: | Windermere, UK | ||||||
| Issuing Agency: | Freshwater Biological Association | ||||||
| Additional Information: | Translated from Russian into English | ||||||
| Uncontrolled Keywords: | Algae; Chlorophylls; Methodology; Biofilters; Light absorption | ||||||
| Subjects: | Biology Ecology Limnology | ||||||
| Item ID: | 4794 | ||||||
| Depositing User: | Hardy B Schwamm | ||||||
| Date Deposited: | 14 Feb 2011 13:55 | ||||||
| Last Modified: | 29 Sep 2011 17:52 | ||||||
| URI: | http://aquaticcommons.org/id/eprint/4794 |
Available Versions of this Item
- Generation time and utilization of energy by aquatic bacteria. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]. (deposited 14 Feb 2011 13:54)
- On a method of determination of chlorophyll in cells of algae collected on a membrane filter. [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 11(14), 198-202, 1966.]. (deposited 14 Feb 2011 13:55)[Currently Displayed]
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