Cultural methods of detection for microorganisms: recent advances and successes

Watkins , J. and Jian, X. (1997) Cultural methods of detection for microorganisms: recent advances and successes. In: Sutcliffe, D.W. (ed.) The microbiological quality of water. Ambleside, UK, Freshwater Biological Association, pp. 19-27. (FBA Special Publications,5)

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Abstract

Most microbiological methods require culture to allow organisms to recover or to selectively increase, and target organisms are identified by growth on specific agar media. Many cultural methods take several days to complete and even then the results require confirmation. Alternative techniques include the use of chromogenic and fluorogenic substances to identify bacteria as they are growing, selective capture using antibodies after short periods of growth, molecular techniques, and direct staining with or without flow cytometry for enumeration and identification. Future microbiologists may not use culture but depend on the use of specific probes and sophisticated detection systems.

Item Type: Book Section
Title: Cultural methods of detection for microorganisms: recent advances and successes
Personal Creator/Author:
CreatorsEmail
Watkins , J.
Jian, X.
Title of Book: The microbiological quality of water
Editors:
EditorsEmail
Sutcliffe, D.W.
Refereed: No
Series Name: FBA Special Publications
Number: 5
Page Range: pp. 19-27
Date: 1997
Publisher: Freshwater Biological Association
Place of Publication: Ambleside, UK
ISBN: 0-900386-57-6
Issuing Agency: Freshwater Biological Association
Uncontrolled Keywords: Water quality; Microorganisms; Methodology; Detection; Bacteria; Cultured organisms; Enzymes; Immunofluorescence; Flow cytometry; Microbiological culture
Subjects: Engineering
Limnology
Pollution
Item ID: 5317
Depositing User: Hardy B Schwamm
Date Deposited: 10 May 2011 13:09
Last Modified: 29 Sep 2011 15:05
Related URLs:
URI: http://aquaticcommons.org/id/eprint/5317

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