Recent progress in the identification and determination of freshwater phytoplankton in the natural environment

Wilhelm, C. and Lohmann, C. (1997) Recent progress in the identification and determination of freshwater phytoplankton in the natural environment. In: Sutcliffe, D.W. (ed.) The microbiological quality of water. Ambleside, UK, Freshwater Biological Association, pp. 81-91. (FBA Special Publications,5)

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Abstract

The biomass of the phytoplankton and its composition is one of the most important factors in water quality control. Determination of the phytoplankton assemblage is usually done by microscopic analysis (Utermöhl's method). Quantitative estimations of the biovolume, by cell counting and cell size measurements, are time-consuming and normally are not done in routine water quality control. Several alternatives have been tried: computer-based image analysis, spectral fluorescence signatures, flow cytometry and pigment fingerprinting aided by high performance liquid chromatography (HPLC). The latter method is based on the fact that each major algal group of taxa contains a specific carotenoid which can be used for identification and relative quantification of the taxa in the total assemblage. This article gives a brief comparative introduction to the different techniques available and presents some recent results obtained by HPLC-based pigment fingerprinting, applied to three lakes of different trophic status. The results show that this technique yields reliable results from different lake types and is a powerful tool for studying the distribution pattern of the phytoplankton community in relation to water depth. However, some restrictions should be taken into account for the interpretation of routine data.

Item Type: Book Section
Title: Recent progress in the identification and determination of freshwater phytoplankton in the natural environment
Personal Creator/Author:
CreatorsEmail
Wilhelm, C.
Lohmann, C.
Title of Book: The microbiological quality of water
Editors:
EditorsEmail
Sutcliffe, D.W.
Refereed: No
Series Name: FBA Special Publications
Number: 5
Page Range: pp. 81-91
Date: 1997
Publisher: Freshwater Biological Association
Place of Publication: Ambleside, UK
ISBN: 0-900386-57-6
Issuing Agency: Freshwater Biological Association
Uncontrolled Keywords: Water supply; Phytoplankton; Identification; Chromatographic techniques; Water quality; Water quality control; Inland water environment; Methodology; Chlorophylls; Germany
Subjects: Ecology
Limnology
Pollution
Item ID: 5326
Depositing User: Hardy B Schwamm
Date Deposited: 11 May 2011 11:56
Last Modified: 29 Sep 2011 15:05
Related URLs:
URI: http://aquaticcommons.org/id/eprint/5326

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