Immunodetection of planktonic algae

Taylor, J.A. (1997) Immunodetection of planktonic algae. In: Sutcliffe, D.W. (ed.) The microbiological quality of water. Ambleside, UK, Freshwater Biological Association, pp. 92-103. (FBA Special Publications,5)

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Interest in the identification and characterisation of cyanobacteria and dinoflagellates in aquatic environments is increasing rapidly due to the perceived roles of these organisms in primary production and nuisance aspects in terms of water treatment and public health. Techniques for the identification and quantification of these organisms currently are limited, and the application of molecular approaches provides fundamental taxonomic information and techniques of practical value. Antigenic properties of algal cells may be useful taxonomic markers. Immunodetection techniques utilise the specificity of the antibody/antigen association as a probe for recognising and distinguishing between microorganisms according to their cell- surface chemistry. Immunofluorescent detection of unicellular cyanobacteria and dinoflagellates has been studied with success in marine and freshwater ecosystems and a range of techniques and results are presented and discussed. The most recent advances in the study of planktonic algae have come with the application of continuous flow cytometric methods (CFC). Flow cytometry makes use of the autofluorescence properties of the algal cells, which alone can be used to demonstrate their presence and permit their quantification in natural water samples. When used in conjunction with immunolabelling techniques, the potential of CFC analysis is broadened to study the serological/strain composition of plankters in natural populations. Changes in algal strains represented within and between waters over periods of time are reported and discussed, along with the ecological issues thus raised.

Item Type: Book Section
Title: Immunodetection of planktonic algae
Personal Creator/Author:
Taylor, J.A.
Title of Book: The microbiological quality of water
Sutcliffe, D.W.
Refereed: No
Series Name: FBA Special Publications
Number: 5
Page Range: pp. 92-103
Date: 1997
Publisher: Freshwater Biological Association
Place of Publication: Ambleside, UK
ISBN: 0-900386-57-6
Issuing Agency: Freshwater Biological Association
Uncontrolled Keywords: Bacteria; Methodology; Algae; Plankton; Immunology; Detection; Toxicity tests; Immunofluorescence; Synechococcus; Alexandrium tamarens; Prorocentrum micans
Subjects: Ecology
Item ID: 5333
Depositing User: Hardy B Schwamm
Date Deposited: 12 May 2011 12:08
Last Modified: 29 Sep 2011 15:04
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