Detection of cryptosporidium oocysts in water and environmental concentrates

Smith, H.V. (1997) Detection of cryptosporidium oocysts in water and environmental concentrates. In: Sutcliffe, D.W. (ed.) The microbiological quality of water. Ambleside, UK, Freshwater Biological Association, pp. 126-138. (FBA Special Publications,5)

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Abstract

Whilst current methods for the isolation and enumeration of Cryptosporidium spp. oocysts in water have provided some insight into their occurrence and significance, they are regarded as being inefficient, variable and time-consuming, with much of the interpretation being left to the expertise of the analyst. Two expectations of novel developments are to reduce the variability and subjectivity associated with the isolation and identification of oocysts. Flocculation, immunomagnetisable and flow cytometric techniques, for concentrating oocysts from water samples, should prove more reliable than current methods, whilst the development of more avid and specific monoclonal antibodies in conjunction with the use of nuclear fluorochromes will aid identification. Further insight into the viability, taxonomy, species identification, infectivity and virulence of the parasite should be forthcoming through the use of techniques such as the polymerase chain reaction, in situ hybridisation and non-uniform alternating current electrical fields. Such information is necessary in order to enable microbiologists, epidemiologists, engineers, utility operators and regulators to assess the safety of a water supply, with respect to Cryptosporidium contamination, more effectively.

Item Type: Book Section
Title: Detection of cryptosporidium oocysts in water and environmental concentrates
Personal Creator/Author:
CreatorsEmail
Smith, H.V.
Title of Book: The microbiological quality of water
Editors:
EditorsEmail
Sutcliffe, D.W.
Refereed: No
Series Name: FBA Special Publications
Number: 5
Page Range: pp. 126-138
Date: 1997
Publisher: Freshwater Biological Association
Place of Publication: Ambleside, UK
ISBN: 0-900386-57-6
Issuing Agency: Freshwater Biological Association
Uncontrolled Keywords: Oocytes; Protozoan diseases; Parasites; Life cycle; Disease transmission; Methodology; Antibodies; Fluorescence microscopy; Luminescence; Protists
Subjects: Ecology
Limnology
Pollution
Item ID: 5340
Depositing User: Hardy B Schwamm
Date Deposited: 12 May 2011 12:01
Last Modified: 29 Sep 2011 15:02
URI: http://aquaticcommons.org/id/eprint/5340

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