Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker

Muchlisin, Z.A. and Masazurah, A.R. and Abu Talib, A. and Siti-Azizah, M.N. and Samsudin, B. and Jamsari, A.F.J. (2009) Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker. In: 8th Malaysia Genetic Congress: Role of Genetics in Wealth Creation and Quality of Life , 4-6 August 2009 ,Awana Genting, Pahang, Malaysia, pp. 74-77.

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Abstract

Random Amplified Polymorphic DNA (RAPD) markers and cytochrome b (Cyt-b) gene sequences were utilized to fingerprint and construct phylogenetic relationships among four species of mackerel commonly found in the Straits of Malacca namely Rastrelliger kanagurta, R. brachysoma, Decapterus maruadsi and D. russelli. The UPGMA dendogram and genetic distance clearly showed that the individuals clustered into their own genus and species except for the Decapterus. These results were also supported by partial mtDNA cytochrome b gene sequences (279 bp) which found monotypic sequence for all Decapterus studied. Cytochrome b sequence phylogeny generated through Neighbor Joining (NJ) method was congruent with RAPD data. Results showed clear discrimination between both genera with average nucleotide divergence about 25.43%. This marker also demonstrated R. brachysoma and R. kanagurta as distinct species separated with average nucleotide divergence about 2.76%. However, based on BLAST analysis, this study indicated that the fish initially identified as D. maruadsi was actually D. russelli. The results highlighted the importance of genetic analysis for taxonomic validation, in addition to morphological traits.

Item Type: Conference or Workshop Item
Title: Genetic identification of four Malaysian mackerel species off Coast of Peninsular Malaysia based on molecular marker
Personal Creator/Author:
CreatorsEmail
Muchlisin, Z.A.muchlisinza@yahoo.com
Masazurah, A.R.
Abu Talib, A.
Siti-Azizah, M.N.
Samsudin, B.
Jamsari, A.F.J.
Page Range: pp. 74-77
Date: 2009
Contact Email Address: muchlisinza@yahoo.com
Event Title: 8th Malaysia Genetic Congress: Role of Genetics in Wealth Creation and Quality of Life
Event Type: Conference
Event Location: Awana Genting, Pahang, Malaysia
Event Dates: 4-6 August 2009
Subjects: Aquaculture
Biology
Fisheries
Item ID: 7532
Depositing User: Dr. Z.A. Muchlisin
Date Deposited: 10 Jan 2012 17:24
Last Modified: 10 Jan 2012 17:24
URI: http://aquaticcommons.org/id/eprint/7532

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