Advances in methods for determining fecundity: application of the new methods to some marine fishes

Witthames, Peter R. and Thorsen, Anders and Murua, Hilario and Saborido-Rey, Francisco and Greenwood, Lorraine N. and Dominguez, Rosario and Korta, Maria and Kjesbu, Olav S. (2009) Advances in methods for determining fecundity: application of the new methods to some marine fishes. Fishery Bulletin, 107(2), pp. 148-164.

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Official URL: http://fishbull.noaa.gov/1072/witthames.pdf

Abstract

Estimation of individual egg production (realized fecundity) is a key step either to understand the stock and recruit relationship or to carry out fisheries-independent assessment of spawning stock biomass using egg production methods. Many fish are highly fecund and their ovaries may weigh over a kilogram; therefore the work time can be consuming and require large quantities of toxic fixative. Recently it has been shown for Atlantic cod (Gadus morhua) that image analysis can automate fecundity determination using a power equation that links follicles per gram ovary to the mean vitellogenic follicular diameter (the autodiametric method). In this article we demonstrate the precision of the autodiametric method applied to a range of species with different spawning strategies during maturation and spawning. A new method using a solid displacement pipette to remove quantitative fecundity samples (25, 50, 100, and 200 milligram [mg]) is evaluated, as are the underlying assumptions to effectively fix and subsample the ovary. Finally, we demonstrate the interpretation of dispersed formaldehyde-fixed ovarian samples (whole mounts) to assess the presence of atretic and postovulatory follicles to replace labor intensive histology. These results can be used to estimate down regulation (production of atretic follicles) of fecundity during maturation.

Item Type: Article
Title: Advances in methods for determining fecundity: application of the new methods to some marine fishes
Personal Creator/Author:
CreatorsEmail
Witthames, Peter R.
Thorsen, Anders
Murua, Hilario
Saborido-Rey, Francisco
Greenwood, Lorraine N.
Dominguez, Rosario
Korta, Maria
Kjesbu, Olav S.
Refereed: Yes
Journal or Publication Title: Fishery Bulletin
Volume: 107
Number: 2
Page Range: pp. 148-164
Date: 2009
ISSN: 0090-0656
Issuing Agency: United States National Marine Fisheries Service
Subjects: Biology
Ecology
Fisheries
Item ID: 8798
Depositing User: Patti M. Marraro
Date Deposited: 11 Jun 2012 18:29
Last Modified: 11 Jun 2012 18:29
URI: http://aquaticcommons.org/id/eprint/8798

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