Microsatellite primers for red drum (Sciaenops ocellatus)

Karlsson, Sten and Renshaw, Mark A. and Rexroad III, Caird E. and Gold, John R. (2008) Microsatellite primers for red drum (Sciaenops ocellatus). Fishery Bulletin, 106(4), pp. 476-482.

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Abstract

In this note, we document polymerase-chain-reaction (PCR) primer pairs for 101 nuclear-encoded microsatellites designed and developed from a genomic library for red drum (Sciaenops ocellatus). Details of the genomic library construction, the sequencing of positive clones, primer design, and PCR protocols may be found in Karlsson et al. (2008). The 101 microsatellites (GENBA NK Accession Numbers EU015882-EU015982) were amplified successfully and used to genotype 24 red drum obtained from Galveston Bay, Texas (Table 1). A total of 69 of the microsatellites had an uninterrupted (perfect) dinucleotide motif, and 30 had an imperfect dinucleotide motif; one microsatellite had an imperfect tetranucleotide motif, and one had an imperfect and compound motif (Table 1 ). Sizes of the cloned alleles ranged from 84 to 252 base pairs. A ‘blast’ search of the GENBANK database indicated that all of the primers and the cloned alleles were unique (i.e., not duplicated).

Item Type: Article
Title: Microsatellite primers for red drum (Sciaenops ocellatus)
Personal Creator/Author:
CreatorsEmail
Karlsson, Sten
Renshaw, Mark A.
Rexroad III, Caird E.
Gold, John R.
Refereed: Yes
Journal or Publication Title: Fishery Bulletin
Volume: 106
Number: 4
Page Range: pp. 476-482
Date: 2008
ISSN: 0090-0656
Issuing Agency: United States National Marine Fisheries Service
Subjects: Biology
Ecology
Fisheries
Item ID: 8828
Depositing User: Patti M. Marraro
Date Deposited: 12 Jun 2012 17:54
Last Modified: 12 Jun 2012 17:54
URI: http://aquaticcommons.org/id/eprint/8828

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