Microsatellite multiplex panels for genetic studies of gray snapper (Lutjanus griseus) and lane snapper (Lutjanus synagris)

Renshaw, Mark A. and Saillant, Eric and Lem, Siya and Berry, Philip and Gold, John R. (2007) Microsatellite multiplex panels for genetic studies of gray snapper (Lutjanus griseus) and lane snapper (Lutjanus synagris). Fishery Bulletin, 105(3), pp. 436-439.

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Abstract

Microsatellites are codominantly inherited nuclear-DNA markers (Wright and Bentzen, 1994) that are now commonly used to assess both stock structure and the effective population size of exploited fishes (Turner et al., 2002; Chistiakov et al., 2006; Saillant and Gold, 2006). Multiplexing is the combination of polymerase chain reaction (PCR) amplification products from multiple loci into a single lane of an electrophoretic gel (Olsen et al., 1996; Neff et al., 2000) and is accomplished either by coamplification of multiple loci in a single reaction (Chamberlain et al., 1988) or by combination of products from multiple single-locus PCR amplifications (Olsen et al., 1996). The advantage of multiplexing micro-satellites lies in the significant reduction in both personnel time (labor) and consumable supplies generally required for large genotyping projects (Neff et al., 2000; Renshaw et al., 2006).

Item Type: Article
Title: Microsatellite multiplex panels for genetic studies of gray snapper (Lutjanus griseus) and lane snapper (Lutjanus synagris)
Personal Creator/Author:
CreatorsEmail
Renshaw, Mark A.
Saillant, Eric
Lem, Siya
Berry, Philip
Gold, John R.
Refereed: Yes
Journal or Publication Title: Fishery Bulletin
Volume: 105
Number: 3
Page Range: pp. 436-439
Date: 2007
ISSN: 0090-0656
Issuing Agency: United States National Marine Fisheries Service
Subjects: Biology
Ecology
Fisheries
Item ID: 8886
Depositing User: Patti M. Marraro
Date Deposited: 18 Jun 2012 09:34
Last Modified: 18 Jun 2012 09:34
URI: http://aquaticcommons.org/id/eprint/8886

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