Li, Zhuozhuo and Gray, Andrew K. and Love, Milton S. and Goto, Akira and Asahida, Takashi and Gharrett, Anthony J. (2006) A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis. Fishery Bulletin, 104(3), pp. 182-196.
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Larval and juvenile rockfishes (Sebastes spp.) are difficult to identify using morphological characters. We developed a key based on sizes of restriction endonuclease fragments of the NADH dehydrogenase-3 and -4 (ND3/ND4) and 12S and 16S ribosomal RNA (12S/16S) mitochondrial regions. The key makes use of variation in the ND3/ND4 region. Restriction endonuclease Dde I variation can corroborate identifications, as can 12S/16S variation. The key, based on 71 species, includes most North American taxa, several Asian species, and Sebastolobus alascanus and Helicolenus hilgendorfi that are closely related to rockfishes. Fifty-eight of 71 rockfish species in our database can be distinguished unequivocally, using one to five restriction enzymes; identities of the remaining species are narrowed to small groups: 1) S. polyspinis, S. crameri, and S. ciliatus or variabilis (the two species could not be distinguished and were considered as a single species) ; 2) S. chlorostictus, S. eos, and S. rosenblatti; 3) S. entomelas and S. mystinus; 4)S. emphaeus, S. variegatus, and S. wilsoni; and 5) S. carnatus and S. chrysomelas.
|Title:||A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis|
|Journal or Publication Title:||Fishery Bulletin|
|Page Range:||pp. 182-196|
|Issuing Agency:||United States National Marine Fisheries Service|
|Depositing User:||Patti M. Marraro|
|Date Deposited:||03 Aug 2012 20:37|
|Last Modified:||03 Aug 2012 20:37|
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