A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis

Li, Zhuozhuo and Gray, Andrew K. and Love, Milton S. and Goto, Akira and Asahida, Takashi and Gharrett, Anthony J. (2006) A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis. Fishery Bulletin, 104(3), pp. 182-196.

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Abstract

Larval and juvenile rockfishes (Sebastes spp.) are difficult to identify using morphological characters. We developed a key based on sizes of restriction endonuclease fragments of the NADH dehydrogenase-3 and -4 (ND3/ND4) and 12S and 16S ribosomal RNA (12S/16S) mitochondrial regions. The key makes use of variation in the ND3/ND4 region. Restriction endonuclease Dde I variation can corroborate identifications, as can 12S/16S variation. The key, based on 71 species, includes most North American taxa, several Asian species, and Sebastolobus alascanus and Helicolenus hilgendorfi that are closely related to rockfishes. Fifty-eight of 71 rockfish species in our database can be distinguished unequivocally, using one to five restriction enzymes; identities of the remaining species are narrowed to small groups: 1) S. polyspinis, S. crameri, and S. ciliatus or variabilis (the two species could not be distinguished and were considered as a single species) ; 2) S. chlorostictus, S. eos, and S. rosenblatti; 3) S. entomelas and S. mystinus; 4)S. emphaeus, S. variegatus, and S. wilsoni; and 5) S. carnatus and S. chrysomelas.

Item Type: Article
Title: A key to selected rockfishes (Sebastes spp.) based on mitochondrial DNA restriction fragment analysis
Personal Creator/Author:
CreatorsEmail
Li, Zhuozhuo
Gray, Andrew K.
Love, Milton S.
Goto, Akira
Asahida, Takashi
Gharrett, Anthony J.
Refereed: Yes
Journal or Publication Title: Fishery Bulletin
Volume: 104
Number: 3
Page Range: pp. 182-196
Date: 2006
ISSN: 0090-0656
Issuing Agency: United States National Marine Fisheries Service
Subjects: Biology
Ecology
Fisheries
Item ID: 8989
Depositing User: Patti M. Marraro
Date Deposited: 03 Aug 2012 18:37
Last Modified: 03 Aug 2012 18:37
URI: http://aquaticcommons.org/id/eprint/8989

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